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In this project we use a novel approach to tackle cancer by combining three molecular targets (p-glycoprotein, protein kinase C and the lysyl oxidase-like 2 enzime) and three synergistic scientific contributions (natural products isolation and identification, biological assays and computational chemistry) to improve upon natural products isolated in-house targeting ABC efflux transporters and PKC, and to develop novel molecular entities able to inhibit the LOXL2 enzyme known to contribute to tumor cell invasion by promoting cytoskeletal reorganization, as a combined strategy to tackle metastatic cancer and multidrug-resistance.
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The project, led and coordinated by University of Veterinary Medicine Budapest (UVMB), aims to address the limited knowledge on the safety aspects of modern foodstuffs, particularly alternative meat products, by examining the levels of process contaminants (acrylamide and PAH) and metals in meat replacement products made from ingredients most often used for meat replacement in marketed products (e.g. wheat, pea, bean, soy) and insect-based products. The study is conducted in Hungary, Sweden, and Portugal to provide a comprehensive assessment across different regions.
Ongoing
2027-12-31
2025-05-21
CONTEMFood
CONTEMFood: Contaminants in Meat Replacement Products
MetCardio_DBC human observational study explores the impact of vegetarian-vegan versus omnivorous dietary patterns and physical activity habits in body composition, skin health and metabolic markers to establish the correlations between these parameters with metabolic health and cardiovascular risk. The following parameters are under investigation:
The eating behaviors and physical activity habits of participants using validated questionnaires; Body composition by Bioimpedance and Dual radiation X-ray Absorptiometry (DEXA); Cutaneous physiology by means of carotenoids tests, Transepidermal Water Loss (TEWL), superficial and deep Epidermal hydration, skin biomechanics characters and skin microcirculation; Biochemical parameters, blood pressure, and microcirculation parameters using the LINX DUG Multi-analyzer, validated semi-automatic oscillometer and Multispectral Optoacoustic Tomography (MSOT) imaging, respectively; Cardiovascular risk using QRISK®3-2018.
Past
2024-12-31
2021-12-01
No Acronym
Dietary Patterns and Body Composition: Implications for Metabolic and Cardiovascular Health
The project was based on the application of the concepts of quality by design (QbD) and process analytical technology (PAT – Process Analytical Technology) to evaluate critical quality parameters and develop an optimized cosmetic formulation with rosmarinic acid. The formulations were developed using a factorial experimental design (DoE – Design of Experiments), characterized physically and physicochemically and evaluated for in vitro antioxidant activity and in vitro photoprotective efficacy, as well as for photostability. The formulations were also characterized for their photoprotective efficacy in vivo, as well as evaluated for their safety.
Past
2020-05-31
2019-12-01
No Acronym
Establishment of safety properties and clinical efficacy of rosmarinic acid in Quality by design (QbD) -optimized dermocosmetic formulations
Nanotechnology (NT) is a generic term for techniques, materials, and devices that operate on a manometric scale and represents one of the most promising technologies of the 21st century; and has been considered a new industrial revolution. Nanoencapsulation of active antioxidant compounds represents a future perspective for the development of innovative photoprotective ingredients. Gelatin nanoparticles have been used to develop modified drug and gene delivery systems, as well as drug carriers to specific target sites in the organism. There is no record in the scientific literature of its use in topical application vehicles, intended for cosmetic use. The proposal of this project involved obtaining innovative gelatin nanostructures (containing rutin) for application in sunscreens. The safety and clinical efficacy of the developed nanotechnology platform were assessed by: in vitro skin penetration of gelatin nanoparticles containing rutin, in vertical diffusion cells, using the human epidermis as a biomembrane model; non-invasive assessment of the biophysical properties of the skin, conducted using skin bioengineering equipment.
Past
2015-01-01
2014-01-01
No Acronym
Establishment of the safety properties and cosmetic clinical efficacy of nanostructured systems based on protein technology associated with rutin
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Cancer is a leading cause of death, mostly due to drug resistance. Homologous recombination is an essential cellular mechanism of ADN repair. Several proteins are involved in this process, including BRCA1 in association with its binding partner BARD1. Alterations in this mechanism are found in several cancers and there is strong evidence that targeting these deficiencies can result in therapeutic benefit.
A defective homologous recombination, resulting from BRCA1/2-mutations, proved to be decisive for the efficacy of PARP inhibitors, which are currently in the front line to treat BRCA-mutated cancers such as triple-negative breast, and advanced ovarian cancers and some pancreatic tumors. This approach is based on synthetic lethality. PARP inhibition results in increased DNA double strand breaks, which are not efficiently repaired by homologous recombination in BRCA1/2-deficient cells, causing genomic instability and thus selective death of cancer cells.
Despite the great success of PARP inhibitors, resistance to these agents has been often reported due to residual BRCA1 activity, highly limiting their clinical use. Thus, dysfunction of the BRCA1 pathway has been considered as an effective strategy to improve the therapeutic efficiency of PARP inhibitors.
Recently, we identified a monoterpene indole alkaloid derivative (BBIT20) that targeted homologous recombination DNA repair defects by disrupting BRCA1-BARD1 heterodimer in triple-negative breast and ovarian cancer cells. It showed promising in vitro and in vivo antitumor activity and interacted synergistically with cisplatin and the PARP inhibitor olaparib. BBIT20 was the first reported disrupter of BRCA1-BARD1 complex and may represent a starting point for the development of new and improved anticancer agents against hard-to-treat cancers that still lack effective therapeutic options.
Therefore, using an innovative approach toward unmet needs not only in triple-negative breast and advanced ovarian cancers but also in pancreatic cancer, this project aims at
i) finding new effective plant-derived indole alkaloids for reversing drug resistance in cancer cells, with deficient BRCA1 pathway, by disrupting BRCA1-BARD1 complex and ii) characterizing the disrupting binding mode of BBIT20 of BRCA1-BARD1 interaction by in silico studies.
Past
2025-02-28
2023-03-01
FCT
IndoleBRCA
Exploiting monoterpene indole alkaloid derivatives for overcoming resistance in BRCA-deficient cancers. In silico characterization of binding modes
Skin is the largest organ of the body, covering an area of 1.5–2 m2 and is affected by several external environmental factors such as ultraviolet radiation, air pollution, smoking. Its main functions are temperature regulation, immunity from microorganisms, maintaining electrolyte balance, and protection from physical injuries, chemical agents and ultraviolet (UV) radiation. Skin ageing is one of the most important phenotypes of ageing and occurs in every layer of the skin, primarily due to the degradation of its components. In the last decades, people pay more and more attention to appearance, and skincare and anti-ageing have become one of the hot spots of world concern. Thus, the skin care market based on natural products continues to see strong growth. In this context, the research of naturally occurring anti-ageing agents is greatly expanding and in recent years numerous plant-derived products have been investigated.
In addition, there is in nowadays efforts in search of value aggregation to sub products of the industrial processes. Dehydroabietic acid (DAA) is a natural diterpene and major component of the rosin fraction of oleoresin produced by conifer species, such as grand fir (Abies grandis) and lodgepo le pine (Pinus contorta). It is a substance present in significant quantities in the paper mill effluents. However, previous studies have demonstrated that DAA has antiaging effects in human dermal fibroblasts.
Skin ageing is closely related with Ultraviolet exposure and as well as skin microcirculation alterations vascular ageing-related. However, there is no investigation about the potential benefits of formulations containing DAA in human skin. Thus, the purpose of this work is to develop nanotechnology-based formulations containing DAA, as well as to evaluate the effects of them on human skin considering biomechanical, hydration, microcirculation aspects, and irritative potential. It is expected that this work will be the first step to allow the use of this substance in the cosmetic formulations, as well as to value DAA which in current is a subproduct in the cellulose industry.
Past
2022-06-30
2021-01-01
No Acronym
Exploiting the potential of dehydroabietic acid formulations to tackle skin ageing
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Barreto","lastName":"Vieira"},"role":{"uri":"/dk/atira/pure/upmproject/roles/upmproject/coi","term":{"en_GB":"Researcher","pt_PT":"Investigador"}},"person":{"systemName":"Person","uuid":"083c53ca-ac88-4f87-b4b3-9e234f1a5990"},"organizations":[{"systemName":"Organization","uuid":"79a7d58b-ba26-401a-8fb7-d5b5606228b8"}]},{"typeDiscriminator":"InternalParticipantAssociation","pureId":36208490,"name":{"firstName":"Íris Cláudia Felisberto","lastName":"Guerreiro"},"role":{"uri":"/dk/atira/pure/upmproject/roles/upmproject/coi","term":{"en_GB":"Researcher","pt_PT":"Investigador"}},"person":{"systemName":"Person","uuid":"6004b193-851f-4d1d-a8c3-dd1d7bdec75c"},"organizations":[{"systemName":"Organization","uuid":"79a7d58b-ba26-401a-8fb7-d5b5606228b8"}]},{"typeDiscriminator":"InternalParticipantAssociation","pureId":36208492,"name":{"firstName":"Sofia Ramos Falcão","lastName":"Lopes"},"role":{"uri":"/dk/atira/pure/upmproject/roles/upmproject/coi","term":{"en_GB":"Researcher","pt_PT":"Investigador"}},"person":{"systemName":"Person","uuid":"6d39fc19-598a-4d98-ad0f-1fa47a393e37"},"organizations":[{"systemName":"Organization","uuid":"79a7d58b-ba26-401a-8fb7-d5b5606228b8"}]}]
Nutrition is a key player in diabetes management, and compounds in our diet, particularly (poly)phenols, could be vital in slowing disease progression.Urolithins, unique molecules produced by gut bacteria, have been found to inhibit IAPP aggregation and its harmful effects on pancreatic β -cells. EXPLORER sets out to investigate the impact of pomegranate supplements, rich in urolithin- precursor compounds, on metabolic markers and β-cell function in individuals at risk of diabetes. To improve urolithin´s effectiveness, an innovative nanotechnology solution is proposed to ensure the molecules reach β-cells. By raising awareness and translating these discoveries into practical dietary and technological innovations, EXPLORER aims to contributes to diabetes prevention and care.
Ongoing
2026-06-30
2023-06-01
https://doi.org/10.62658/FAZER/ILIND/CBIOS/1/2023
EXPLORER
Exploring Unconventional Plant-Derived Metabolites to Keep Away Diabetes: The case of Pomegranate
The members of the human TMBIM protein family are highly conserved intracellular ion channels that affect cancer progression-related cell processes. The correlation between the expression levels of these proteins and cancer patient survival rates demonstrates the significance of these proteins in cancer progression. Despite representing appealing targets for anti-cancer progression therapy, there are no TMBIM pharmacological modulators identified. This project aims at generating in silico models of the human sequences for these proteins and to construct various systems comprising the human TMBIM (hTMBIM) channel pore embedded in a lipid bilayer to perform molecular dynamics studies. This approach will allow later the discovery of active molecules by performing high throughput screens of commercially available molecules able to modulate the ion conductivity of TMBIM. This project aims at providing fundamental information regarding the gating mechanism controlling the ion flow through TMBIM channels, and to discover the first putative pharmacological modulators of these proteins.
Past
2022-01-01
2021-01-01
FCT
TMBIM
Fine-tuning TMBIM ion channel activity to control hallmarks of cancer
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The PT -ASAE authority has consistently gathered and provided data on the safety of plant-based food supplements marketed in Europe and cooperated through Briefing Notes to its discussion as an issue of concern since the creation of EFSA (see Botanicals | EFSA (europa.eu)), and during the Emerging Risks Exchange Network (EREN) meeting in April 2021. Thus, it is greatly welcome the opportunity EFSA launched to a project for an outsourced activity that was developed by the SCER/KNOW Secretariat included under NEXT Programme, further endorsed at the Advisory Forum (AF) in March 2023.
After the kick-off meeting on 29th January, the PT -ASAE Group is actively focused on contributing to the establishment of a topic community of knowledge on food supplements, in strategic partnership, engaging with expertise for signals in nutrivigilance systems and for analyzing plant-based substances of concern.
It is also clear that the community’s role is to identify possible emerging risks without mandate to conduct risk assessment as per Regulation (EC) 178/2002 or Reg. (EC) 1925/2006.
Task 1 - Work Package 1: Establishment and moderation of Community of Knowledge on food supplements other than vitamins and minerals
Task 2 - Work Package 3: Identifying potentially hazardous food supplements, other than vitamins and minerals, containing plant-based substances with predicted toxicity in the EFSA Compendium on Botanicals
Past
2026-01-01
2024-01-01
No Acronym
Identification of emerging risks associated with food supplements, other than vitamins and minerals
